The Gadsden flag, providing a rattlesnake and the phrase "Don't Tread on Me

https://cruzbuxy751.solsticebrief.com/posts/the-ultimate-guide-to-buying-historic-civil-war-flags-online-4

The Gadsden flag, offering a rattlesnake and the word "Don't Tread on Me," was once adopted by way of some Confederate items as a symbol of resistance in opposition to perceived oppression.